Catalog Number - 85118-10P-2

The 85118‑10P‑2 Cogent RP C18 ABX™ UHPLC Column is engineered for high‑resolution separations of compounds with extreme pKa values, providing stable, reproducible performance across a wide pH range (2–12).

Featuring bulky end‑capping and high‑purity silica bonding, this column minimizes secondary interactions and enhances peak shape for acidic, basic, and polar analytes under demanding mobile phase conditions.

✅ Ideal as a USP L1 alternative for methods involving strong acids or bases

100 mm Length – Increased Resolution for LC‑MS Methods

• Higher resolving power than 50 mm columns

• Improved separation of closely related compounds

• Increased peak capacity for complex mixtures

• Ideal for method refinement after initial screening

✅ Selected when greater resolution is required beyond fast scouting methods

2.1 mm ID – LC‑MS / Low‑Flow Optimization

• Designed for UHPLC and LC‑MS systems

• Enhances MS sensitivity with reduced solvent flow

Typical Flow Rate Range

• ~0.2–0.3 mL/min

✅ Requires UHPLC systems capable of stable low‑flow performance

1.8 µm Particle – Maximum Efficiency & Performance

• Delivers ultra‑high efficiency and sharper peak shapes

• Greater peak capacity vs 3 µm particles

• Ideal for complex sample matrices and trace analysis

✅ Enables high‑performance LC‑MS and UHPLC workflows

⚠️ Higher backpressure expected — UHPLC system required

Wide pH Stability (pH 2–12) – Extreme Conditions Capability

• Supports acidic and basic mobile phases

• Suitable for:

  • Strong acids

  • Strong bases

• Maintains performance under aggressive method conditions

✅ Enables method flexibility beyond standard C18 columns

Why Choose RP C18 ABX™ Columns

Extreme pH Ruggedness

• Stable across full pH spectrum (2–12)

• Ideal for challenging analytes and harsh conditions

Improved Peak Shape for Acids & Bases

• Specialized end‑capping reduces secondary interactions

• Enhances reproducibility and accuracy

Efficient Method Development

• Faster runs reduce solvent consumption and cost

• Supports higher instrument throughput

✅ Excellent for advanced method development and LC‑MS optimization

System & Sample Considerations

• ⚠️ Requires UHPLC system capable of high pressure operation

• Recommended sample preparation:

  • 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column clogging

• Maintain performance and longevity

Typical Applications

• LC‑MS and UHPLC analysis

• Acidic and basic compound separations

• Pharmaceutical method development

• Stability‑indicating methods

• Peptide and polar compound analysis

• Complex mixture resolution under extreme pH

Important Usage Notes

• Designed for UHPLC / LC‑MS workflows

• Requires low‑flow capability (~0.2–0.3 mL/min)

• Best suited for extreme pH mobile phase conditions

• Monitor system pressure to ensure safe operation